PCR Fluorescence Filters

Name: PCR Fluorescence Filters
Substrate material: Optical Glass
Smoothness: 80-50 MIL-C-48497A
Clear aperture: >90%
Edge chamfer: <0.2mm
Operating temperature: -40°C /+180°C (low stress state)
Storage environment: Constant temperature ~23°C; Constant humidity <40%

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PCR fluorescence filter (import replacement)

Polymerase Chain Reaction PCR

FAM, HEX, ROX, CY5 (conventional)

CY5.5, CY3 (extended)

Borisun Optics develops high-quality PCR filter sets based on PCR instruments and fluorescence detection, providing key filter components for PCR equipment and providing an alternative solution to solve my country's dependence on imported fluorescence filter sets.

After years of accumulation of fluorescence filter production processes and technologies, and a wealth of experience in R&D, production and application, Borisun Optics has joined hands with PCR instrument manufacturers to officially launch a six-channel high-quality PCR-specific filter set after repeated improvements and verifications.

The PCR-specific filter set provided by Borisun Optics has the characteristics of being able to replace imports, high OD6 cutoff depth, high transmittance of 95%, high cross-cutoff, good transition band steepness, high signal-to-noise ratio, good stability, and long life.

This series of products is used for excitation source applications of LED light sources and laser light sources, excites fluorescent dyes, and generates PCR fluorescent signals. According to the number of channels used by the instrument manufacturer, the channels can be sold separately, or a full set of six-channel filter sets can be purchased.

(If the fluorescence filter set is used for a wide spectrum light source, such as a halogen lamp emission source, please consult Borisun Optics)

Each set contains an excitation filter, a reflection filter, and a dichroic mirror.

The four sets of PCR filter sets for routine inspection are: FAM, HEX, ROX, CY5

The extended six-channel PCR filter set is: FAM, HEX, ROX, CY5, CY5.5, CY3


Channel FAM Application:

Sub-model NameGeneral DimensionsParameters
BP475-33-YG-AFluorescence FiltersD25X3.5mm475-33-OD6-T95-200-800
BP525-20-YG-AFluorescence FiltersD25X3.5mm525-20-OD6-T95-200-800
FLU-LP490Long Pass Dichroic Mirror25.2X35.6X1.1mm45-HR400-480-HT510-750

Excitation and emission fluorescence filter parameters: 475-33-OD6-T90-200-800

Meaning; center wavelength 475nm; bandwidth 33nm; cut-off depth OD6; transmittance 95%; cut-off range 200-800nm

Dichroic mirror parameters: 45-HR400-480-HT510-750

Meaning: incident angle 45 degrees; HR400-480 reflection band 400-480nm; HT510-750 transmission range 510-750nm; transmittance and reflectance 90%

BP 457nm.jpg



Channel HEX Application:

Sub-model NameGeneral DimensionsParameters
BP525-20-YG-AFluorescence FiltersD25X3.5mm525-20-OD6-T95-200-900
BP575-25-YG-AFluorescence FiltersD25X3.5mm575-25-OD6-T95-200-900
FLU-LP550Long Pass Dichroic Mirror25.2X35.6X1.1mm550LPF560-750HT480-535HR

BP 525nm.jpg


Channel ROX Application:
Sub-model NameGeneral DimensionsParameters
BP575-25-YG-AFluorescence FiltersD25X3.5mm575-25-T95-OD6-200-900
BP625-30-YG-AFluorescence FiltersD25X3.5mm625-30-T95-OD6-200-900
FLU-LP595Long Pass Dichroic Mirror25.2X35.6X1.1mm590LPF610-700HT500-580HR
bp 575nm.jpg

Channel CY5 Application:

Sub-model NameGeneral DimensionsParameters
BP625-30-YG-AFluorescence FiltersD25X3.5mm625-30-T95-OD6-200-900
BP692-45-YG-AFluorescence FiltersD25X3.5mm692-45-T95-OD6-200-900
FLU-LP595Long Pass Dichroic Mirror25.2X35.6X1.1mm660LPF670-800HT600-640HR

bp 625nm.jpg


Channel CY5.5 Application-Extended Channel:

Sub-model NameGeneral DimensionsParameters
BP665-15-YG-AFluorescence FiltersD25X3.5mm665-15-OD6-T95-200-800
BP715-20-YG-AFluorescence FiltersD25X3.5mm715-20-OD6-T95-200-800
FLU-LP690Long Pass Dichroic Mirror25.2X35.6X1.1mm45-HR665-HT715
BP 665NM.png

Channel CY3 Application-Extended Channel:

Sub-model NameGeneral DimensionsParameters
BP543-20-YG-AFluorescence FiltersD25X3.5mm543-20-OD6-T95-200-800
BP585-20-YG-AFluorescence FiltersD25X3.5mm585-20-OD6-T95-200-800
FLU-LP545Long Pass Dichroic Mirror25.2X35.6X1.1mm550LPF560-750HT480-535HR

BP 543NM.png


This series of fluorescence filter sets, in addition to PCR research and equipment applications, can also be used in genetics, biology, immunology, imaging fluorescence, etc., and can be adapted to achieve good results.

Fluorescence filters or filter sets of other sizes and specifications can be customized.

If you have any questions, please feel free to contact our professional engineers to answer your questions.

If you are interested in this product and want to know more detailed product information, please fill in the following form to contact the manufacturer directly:

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